Mid-1800s - Beginning research of Embryo
1908 – ‘StemCell’ from Alexander Maksimov
1963 – Self reproducing cell
1978 – Found Blood stemcell
1981 - Embryonic stemcell
1998 - Human's embryonic stemcell
2001 – First human embryo cloning
(중략)
2.1 Adult StemCell - How?From Bone Marrow
HSCs and MSCs are rich in b
is found in many tissues in several alternatively spliced forms. The LEP gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
명칭
Leptin
구성
16kDa Protein
분비 장소
Adipose Tissue(지방세포)
유전 정보 위치
사람 : 7번 염색체, 쥐 : 6번 염색체
역할
호르몬으로써 시상하부로 가서 여러 가지 기작을 조절.
Stemcell calssification
1. Based upon location
Embryonic stemcells
Somatic/ adult stemcells
2. Based upon function
Totipotent
Pluripotent
Multipotent
Unipotent
Reject Response
Traditional Immuno-repressents
Constant administration
Little engraftment after 3~4 weeks
Host immune system impaired
Vulnerable to Infection
↓Need brief immuno-re
3. 암화에 의한 세포주 확립
동물세포를 이용한 연구 발전에 기여
4. Sato등에 의해 무혈청 배양법 확립
1960. 무혈청 배양 시도
1963-5(Ham) 혈청 공급 없이
CHO cell cloning
1970(Sato) 혈청 대신 호르몬
첨가 cell line 무혈청 배양
성공
Human embryonal kidney, SV40 and large T antigen transformed